Polypeptide Composition and Amino-Terminal Sequence of Broad Bean Polyphenoloxidase

نویسندگان

چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Polypeptide composition and amino-terminal sequence of broad bean polyphenoloxidase.

Polyphenoloxidase was purified from broad bean (Vicia faba) leaves. The purified enzyme contained two immunocross-reactive proteins of approximately 60 to 65 and 43 to 45 kilodaltons. Further electrophoretic separation resolved these proteins into doublets with molecular mass of 61.5, 60, 44.5, and 43 kilodaltons, respectively. Each of the four polypeptides was transferred to Immobilon and subj...

متن کامل

Amino acid composition and amino-terminal sequence of yeast enolase.

The recent finding (1, 2) that part of the enolase molecule can be removed by digestion with leucine aminopeptidase or carboxypeptidase without change in enzymic activity has prompted a detailed study of the relation between structure and activity in this enzyme. As a basis for such investigations it is necessary to have a knowledge of the principal features of enolase structure; the amino acid...

متن کامل

The amino acid sequence of plastocyanin from Vicia faba L. (broad bean).

The amino acid sequence of plastocyanin from broad bean was determined. It consists of a single polypeptide chain of 99 residues. The sequence was determined by using a Beckman 890C sequencer and by dansyl-phenyl isothiocyanate analysis of peptides obtained by the enzymic cleavage of purified cyanogen bromide fragments. Some parts of the sequence depend on the results of Edman degradation of pe...

متن کامل

Subtilisin Inhibitor from Seeds of Broad Bean (vicia Faba); Purification, Amino Acid Sequence and Specificity of Inhibition

A potent inhibitor of microbial serine proteases, including subtilisin, has been purified 1100-fold from seeds of broad bean (Vicia faba, cv. Kleine Thtiringer). Chymotrypsin and trypsin were not inhibited, but a weak "temporary" inhibition of pancreas elastase was observed. The preparation of pure inhibitor contained one major molecular form with a blocked N-terminal (MW 10,000, pl 4.8) and a ...

متن کامل

The amino-terminal sequence of porcine pepsinogen.

Amino acid sequence studies were performed on (a) peptides obtained from tryptic hydrolysates of succinyl pepsinogen and (b) those isolated from a peptide mixture formed when pepsinogen is activated to pepsin. These peptides taken together with tryptic peptides obtained from reduced carboxymethylated pepsinogen establish the complete sequence of 41 residues at the NH&erminal end of the single p...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Plant Physiology

سال: 1989

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.91.2.481